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KMID : 0903519990420030130
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1999 Volume.42 No. 3 p.130 ~ p.133
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Lipoxygenase Activity in Black Rices
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Lee, You Seok
Song, Seon Joo/Rhee, Chong Ouk
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Abstract
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Lipoxygenase activities of three cultivars of black rice (Chindo, Suwon-415, Yongkeum-1) were determined using an oxygen polargraphic method to measure oxygen uptake. Studies at different pH levels revealed that the optimum pH was about pH 7.0 for Suwon-415 and pH 7.5 for Chindo and Yongkeum-1. The specific activities of Chindo, Suwon-415 and Yongkeum-1 at optimal pH were 41.0, 27.3, and 29.6 unit/㎎-protein, respectively. In all the cultivars, there was an increase in the activity with increase in reaction temperature. Enzyme activity was tested at different concentrations of the substrate. The resulting K_m and V_(max) values of Chindo, Suwon-415, and Youngkeum-1 were 0.059, 0.050, and 0.066 mM and 2020, 2283, and 1616 unit/g-grain, respectively. Enzyme activity decreased at all storage temperatures(25, 4, and -40℃).
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